Table V.
Summary of the Discrepant Results (Highlighted in Bold Type) Between the In‐House Real‐Time PCR and Seeplex® RV12 (a) and RV15 (b)
| Sample # | PCR result (Ct) | RV12 |
|---|---|---|
| (a) | ||
| 7 | FLB (35.77) | None |
| 29 | FLB (35.70) | None |
| 83 | FLB (32.01) | None |
| 94 | FLB (37.56) | None |
| 60 | HRV (33.54) | None |
| 43 | MPNV (35.32) | None |
| 32 | PF1 (35.21) | RSVb |
| 27 | HRV (29.34), MPNV (35.62) | HRV, ADV |
| 82 | FLA (30.81), MPNV (35.94) | FLA |
| 87 | MPNV (26.57), PF3 (33.92) | MPNV |
| 100 | PF2 (28.5), ADV (38.11) | PF2 |
| 17 | ADV (31.65) | ADV, 229E |
| 18 | ADV (26.85), RSV (32.64) | ADV, RSVa, HRV |
| 39 | RSV (30.24) | RSVa, OC43, ADV |
| 47 | ADV (33.44) | ADV, OC43 |
| 50 | FLA (28.95) | FLA, HRV |
| 55 | HRV (32.69) | HRV, OC43 |
| 66 | HRV (21.16) | HRV, RSVa |
| 70 | FLA (27.98) | FLA, RSVa |
| 71 | HRV (31.81) | HRV, RSVa |
| 90 | PF3 (31.81) | PF3, RSVb |
| 102 | PF2 (29.14) | PF2, HRV |
| Sample # | PCR result (Ct) | RV15 |
|---|---|---|
| (b) | ||
| 156 | FLB (26.50) | None |
| 158 | FLB (30.30) | None |
| 159 | FLB (30.41) | None |
| 160 | FLB (29.46) | None |
| 170 | FLB (31.64) | None |
| 142 | ADV (30.02) | None |
| 143 | ADV (29.76) | None |
| 150 | ADV (23.38) | None |
| 108 | PIV‐2 (26.98), FLA (28.70) | None |
| 182 | ADV (27.20) | HRV, RSV |
| 107 | MPNV (26.92) | MPNV, HRV |
| 134 | RSV (29.86) | RSVb, HEV |
| 165 | FLA (27.03) | FLA, OC43 |
| 176 | FLB (25.13) | FLB, OC43 |
| 183 | RSV (31.96) | RSVb, BocV |
Samples where Seeplex® missed targets detected by PCR are given first.