. 2012 Aug 15;84(10):1646–1651. doi: 10.1002/jmv.23375

Table II.

Primers and Probes Used in This Study

Primers/probe	Sequence 5′–3′	Gene target	Orientation	Nucleotide position
Influenza A
Forward primer	GGAATGGCTAAAGACAAGACCAAT	Matrix protein	Forward	129–152^a
Reverse primer	GGGCATTTTGGACAAAGCGTCTAC	Matrix protein	Reverse	250–227^a
Probe	FAM‐AGTCCTCGCTCACTGGGCACGGTG‐BHQ1	Matrix protein	Reverse	221–198^a
Influenza B
Forward primer	CCAGGGATTGCAGACATTGA	Nucleoprotein	Forward	928–947^b
Reverse primer	ACAGGTGTTGCCATATTGTAAAGAG	Nucleoprotein	Reverse	1,118–1,094^b
Original probe	Yellow Yakima‐TTGTTAGGCCCTCTGTGGCRAGCA‐BHQ1	Nucleoprotein	Forward	980–1,003^b
Modified probe	Yellow Yakima‐TTGTTAGACCTTCTGTGGCRAGCA‐BHQ1	Nucleoprotein	Forward	968–991^c

Based on influenza A/California/07/2009(H1N1), GenBank accession: FJ969537.

Based on influenza B/North Carolina/WRAIR1582P/2009, GenBank accession: CY069566.

Based on influenza B/Singapore/1/2011, GenBank accession: CY093580.