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. 2016 Sep 9;89(4):672–684. doi: 10.1002/jmv.24674

Figure 2.

Figure 2

Overexpression of IFIT1 did not regulate HCMV AD169 replication in HELFs. A1 and A2, transduction efficiency by FCM. A1, non‐transduced astrocytes; A2, lv/IFIT1/EGFP transduced astrocytes after 48 hr; A3, non‐transduced HELFs; A4, lv/IFIT1/EGFP transduced HELFs after 48 hr. B1–B12, cell morphology observation under con‐focal microscope. B1, B2, B5, and B6, lv/IFIT1/EGFP transduced astrocytes; B3 and B4, lv/ctl/EGFP transduced astrocytes; B7, B8, B11, and B12, lv/IFIT1/EGFP transduced HELFs; B9 and B10, lv/ctl/EGFP transduced HELFs. C1–C3, IE1, IE2, and pp65 by RT‐qPCR C4, Virus titer of supernatant at 72 and 96 hr p.i; C5, intracellular virus titer at 72 and 96 hr p.i. Lv/IFIT1/EGFP, IFIT1 lentivirus; lv/ctl/EGFP, lentivirus negative control; HCMV(+), HCMV infected; HCMV(−), mock infected; H p.i, hours post infection.