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. 2016 Sep 9;89(4):672–684. doi: 10.1002/jmv.24674

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© 2016 Wiley Periodicals, Inc.

This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.

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Knockdown of IFIT1 increased HCMV replication. A1, mRNA of ifit genes was determined by RT‐qPCR with treatment of shIFIT1‐1, shIFIT1‐2, or shIFIT1‐3; A2, protein expression of IFIT1 after treatment with lv/shRNA‐1/EGFP; B1–B3, mRNA expression of IE1, IE2, and pp65 by RT‐qPCR. C, protein expression of IE1, IE2, and pp65 after treatment with lv/shIFIT‐1/EGFP; D1, virus titer of supernatant; D2, intracellular virus titer. E, MTT analysis. HCMV−, mock infected astrocytes, HCMV+, HCMV infected astrocytes; *, statistical significance P < 0.05.