Table 3.
The sensitivity of the RT‐LAMP assays was tested using serial dilutions of viral RNA from several RV‐C species
| Types | Virus isolate names | Number of positive replicates/number of tests for each assayviral RNA concentration (copies/reactions)a | ||||||
|---|---|---|---|---|---|---|---|---|
| Set 1 | Set 2 | |||||||
| 105 | 104 | 103 | 105 | 104 | 103 | 102 | ||
| C02 | 0095‐OsakaC‐JPN‐2016 | 1/3 | 0/3 | 0/3 | NT | 3/3 | 0/3 | 0/3 |
| C06 | 0060‐OsakaC‐JPN‐2016 | 0/3 | 0/3 | 0/3 | NT | 3/3 | 3/3 | 0/3 |
| C09 | 47‐SGH‐JPN‐2015 | 0/3 | 0/3 | 0/3 | NT | 3/3 | 3/3 | 1/3 |
| C12 | 0153‐OsakaC‐JPN‐2016 | 1/3 | 0/3 | 0/3 | NT | 3/3 | 3/3 | 0/3 |
| C18 | 57‐SGH‐JPN‐2014 | 0/3 | 0/3 | 0/3 | NT | 3/3 | 1/3 | 0/3 |
| C23 | 0105‐OsakaC‐JPN‐2016 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | NT | NT |
| C40 | 63‐SGH‐JPN‐2016 | NT | 0/3 | 0/3 | 3/3 | 1/3 | 0/3 | NT |
| C53 | 04‐SGH‐JPN‐2015 | 0/3 | 0/3 | 0/3 | NT | 3/3 | 3/3 | 2/3 |
Abbreviations: NT, not tested; RT‐LAMP, reverse transcription loop‐mediated isothermal amplification; RV, rhinovirus.
a
Viral RNA copy number was calculated based on the 5′‐untranslated regions of each viral gene as described in the text.
This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.