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Stimulation of PBMCs and neutrophils by LPS and RSV in vitro. Human PBMC of healthy volunteers (n = 4) were stimulated with LPS (1 ng/ml) or RSV A2 (MOI 1) and MMP‐8 and MMP‐9 concentrations in supernatant were measured after 24 by ELISA (A) or at transcriptional level by q‐PCR (B). Neutrophils were stimulated for 4 hr and MMP levels were determined in the supernatant (C).
