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. 2009 Jul 7;108(2):415–423. doi: 10.1002/jcb.22268

Figure 1.

Figure 1

Effects of actinomycin (act)‐D‐ or cis‐diamminedichloroplatinum (cis‐DDP) treatment upon rRNA and β‐actin mRNA synthesis in uninfected and HCMV‐infected MRC5 cells. Both uninfected (A,B) and HCMV‐infected (A′,B′) MRC5 human embryo fibroblasts were untreated or act‐D‐, or cis‐DDP‐treated before total RNA extraction and RT‐PCR assay. The time points considered were: 2 h 30 min and 4 h 30 min. A,A′: RT‐PCR amplification pattern (1, 2, 3: 25, 30, and 35 cycles) of a 293 bp RT product, using specific primers for the rRNA ITS1 intronic region. B,B′: RT‐PCR amplification pattern (1, 2, 3: 30, 35, and 40 cycles) of a 242 bp RT product, using specific primers for the third intron of β‐actin mRNA.