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. 2011 May 12;83(7):1121–1127. doi: 10.1002/jmv.22101

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Copyright © 2011 Wiley‐Liss, Inc.

This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.

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Endpoint fluorescence plot of H275Y RT‐PCR assay using clinical specimens. Relative H275 (VIC) and Y275 (FAM) fluorescence are plotted on the y‐axis and x‐axis, respectively. Clinical specimens discriminated as H275 or Y275 are represented as open circles or filled circles, respectively. Viral titers of 3.16 × 10¹, 3.16 × 10⁰, 3.16 × 10⁻¹, and 3.16 × 10⁻² TCID50/reaction of A/Denmark/524/2009 (PC H275, open diamond) and A/Denmark/528/2009 (PC Y275, filled diamond) were used as positive controls for the assay.