Table I.
Primers and Probes
| Names | Sequences (5′–3′) | Orientation | Positiona |
|---|---|---|---|
| Primers for RT‐PCR | |||
| H1N1NA‐F690‐719 | ATGTGCATGTGTAAATGGTTCTTGCTTTAC | + | 690–719 |
| H1N1NA‐R847‐872 | ACACATGTGATTTCACTAGAATCAGG | − | 847–872 |
| Probes for RT‐PCR | |||
| FAM‐274Ya‐swH1N1‐F823‐835 (for detecting resistant virus) | (FAM)TACTATGAGGAAT(MGB) | + | 823–835 |
| VIC‐H274a‐swH1N1‐F823‐835 (for detecting susceptible virus) | (VIC)CACTATGAGGAAT(MGB) | + | 823–835 |
| Primers for sequencing | |||
| N1‐676‐694F | ACACAAGAGTCTGAATGTG | + | 676–694 |
| N1‐1130‐1111R | GGATCCCAAATCATCTCAAA | − | 1130–1111 |
a
The nucleotide positions of NA genes are based on cRNA sequences obtained from GISAID database. Isolate ID numbers for NA genes of A/Denmark/524/2009 and A/Denmark/528/2009 are EPI_ISL_33836 and EPI_ISL_33837.