Table II.

Detection Limits of RT‐PCR Assays Using Serial Dilutions of Viral RNA

Viral titer (TCID50/reaction)	A/Denmark/524/2009			A/Denmark/528/2009
	No. of positive replicates/No. of tests for each assay (positive %)			No. of positive replicates/No. of tests for each assay (positive %)
	Type A rRT‐PCR	H275Y RT‐PCR		Type A rRT‐PCR	H275Y RT‐PCR
		H275	Y275		H275	Y275
3.16 × 10−1	6/6 (100)	6/6 (100)	0/6 (0)	6/6 (100)	0/6 (0)	6/6 (100)
3.16 × 10−2	6/6 (100)	6/6 (100)	0/6 (0)	6/6 (100)	0/6 (0)	6/6 (100)
3.16 × 10−3	2/6 (33.3)	2/6 (33.3)	0/6 (0)	3/6 (50)	0/6 (0)	3/6 (50)
3.16 × 10−4	0/6 (0)	0/6 (0)	0/6 (0)	0/6 (0)	0/6 (0)	0/6 (0)