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. 2016 Dec 23;89(4):571–581. doi: 10.1002/jmv.24571

Figure 3.

Figure 3

Roles of CBX on DENV viral RNA and viral protein synthesis. A549 cells were treated with 100 µM CBX from 1 hr prior to DENV‐1 or DENV‐2 infection till 24 hr post‐infection. a and b: The total RNA was isolated from DENV‐1(a)‐ or DENV‐2(b)‐infected cells and analyzed by quantitative RT‐PCR. c and d: The expression of DENV‐1(c) or DENV‐2(e) viral protein was determined by immunofluorescence assay using DENV prM glycoprotein antibody, and the percentages of DENV‐1(d) or DENV‐2(f) prM containing cells were determined by PicCnt 100×. Data are shown as mean ± SEM of at least three independent experiments. NS, non‐significant by t‐test (n = 3).