Figure 6.

Infectivity of DENV‐2 in A549 cells is inhibited by CBX‐incubation. DENV‐2 particles were pre‐incubated with 100 µM CBX at 37°C for 1 hr, and then infected A549 cells for indicated time. a: The total RNA was isolated from the infected cells and analyzed by quantitative RT‐PCR using primers specific for the viral C protein (CP) mRNA. b: After 24 hr of infection, the expression of viral protein was determined by immunofluorescence assay using DENV prM glycoprotein antibody; c: the percentages of prM containing cells were determined by PicCnt 100×; d: the titer of progeny DENV‐2 particles was measured by TCID₅₀ assay. Data are shown as mean ± SEM of at least three independent experiments. ***P < 0.001.