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. 2016 Dec 23;89(4):571–581. doi: 10.1002/jmv.24571

Figure 7.

Figure 7

The virucidal effects of CBX against DENV, and other enveloped virus. a: A549 cells were treated with indicated concentrations of CBX from 1 hr prior to DENV‐2 infection till 24 hr post‐infection, and the expression of viral RNA from progeny virions in the supernatants was determined by quantitative RT‐PCR. b–d: A549 cells were treated with 100 µM CBX from 1 hr prior to DENV‐2 infection (MOI of 0.1) till 48 hr post‐infection. b: The total RNA was isolated from the infected cells and analyzed by quantitative RT‐PCR. c: The expression of viral protein was determined by immunofluorescence assay using DENV prM glycoprotein antibody. d: The percentages of prM containing cells were determined by PicCnt 100×. e and f: VSV was directly incubated with indicated concentrations of CBX for 6 hr (e) or for indicated time (f) at 37°C. The titer of residual virus was determined by plaque assay. Data are shown as mean ± SEM of at least three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001.