Figure 2.
In vitro transfection efficiency of lipid formulations with different lipids in the presence of mucosal destabilizers for RPMI 2650 (A) and H1299 (B) cells. Emulsions and liposomes were complexed with 0.5 µg of pCMV‐Luc+ at C/Dopt, and then applied to cells in the presence of heparin sulfate (25 equivalency), lung surfactant (2.5 µg), or DNase I (0.5 U/ml). Naked DNA was used as a negative control. Twenty‐four hours after transfection, the cell lysates were prepared in lysis buffer and analyzed for luciferase activity by luminescence assay. Data are expressed as the mean ± SEM (n = 3) in terms of luciferase content (nanograms of luciferase/well)