Figure 3.

In vivo transfection efficiency of commercial liposomes (A) and the liposomes (B) and emulsions (C). pCMV‐Luc+ (6.7 µg) was complexed with various lipid formulations at C/D_opt. Naked DNA was used as a control. Twenty‐four hours after intranasal instillation, nasal and lung lysates were prepared in lysis buffer and analyzed for luciferase activity by luminescence assay. Data are expressed as the mean ± SEM (n = 3) in terms of luciferase concentration (picograms luciferase per milligram total protein)