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. 2020 Apr 16;8:e8888. doi: 10.7717/peerj.8888

Table 1. Gene expression levels in roots of mycorrhizal wild-type (WT) and spr2 mutant tomato plants.

The expression levels1 shown were obtained from non-mycorrhizal roots and mycorrhizal roots colonized for 32, 45 and 50 dpi.

Colonization period 32 dpi 45 dpi 50 dpi
Genotype/Treatment WT (C)2 WT (M)3 WT (M/C)4 spr2 (C) spr2 (M) spr2 (M/C) WT (C) WT (M) WT (M/C) spr2 (C) spr2 (M) spr2 (M/C) WT (C) WT (M) WT (M/C) spr2 (C) spr2 (M) spr2 (M/C)
Gene/Gene category Mycorrhizal colonization marker
LePT4 0.008 4.46 5606 0.003 4.76 1746 0.02 2.52 140 0.004 1.73 403 0.001 1.97 2377 0.003 0.50 185
Brassinosteroids
DET 2 0.55 0.24 0.447 0.27 0.66 2.45 0.70 0.29 0.42 0.28 0.30 1.09 1.15 2.16 1.88 0.81 0.69 0.85
DWF4 0.27 0.14 0.53 0.07 0.27 3.77 0.10 0.02 0.22 0.05 0.10 2.03 0.31 0.10 0.32 0.24 0.25 1.04
CPD 0.34 0.15 0.45 0.16 0.32 2.01 0.15 0.08 0.55 0.12 0.03 0.23 0.16 0.17 1.08 0.10 0.17 1.79
Gibberellins
CPS 0.003 0.05 14.30 0.004 0.03 8.30 0.02 0.07 4.17 0.012 0.006 0.48 0.01 0.10 7.80 0.009 0.011 1.21
KS 0.47 0.17 0.37 0.30 0.33 1.07 0.27 0.24 0.90 0.36 0.28 0.78 0.69 0.39 0.56 0.43 0.52 1.23
GA3ox 0.01 0.33 23.6 0.02 1.09 69.0 0.03 0.39 14.1 0.03 0.45 15.4 0.02 0.68 29.9 0.01 0.26 32.7
GAI 1.63 0.53 0.33 1.14 6.17 5.39 0.65 0.37 0.57 2.91 2.18 0.75 48.6 13.9 0.29 14.9 25.5 1.71
Abscisic acid
TAS14 –5 – – – – – – – – – – – 248 375 1.51 444 746 1.68
NCED1 – – – – – – – – – – – – 0.99 1.96 1.99 1.29 1.92 1.49
Apocarotenoids
CCD7 0.01 0.12 10.9 0.01 0.24 33.6 0.04 0.27 6.81 0.03 0.42 16.7 0.14 0.79 5.51 0.09 0.19 2.10
CCD1a – – – – – – – – – – – – 1.34 0.94 0.70 0.94 0.98 1.04
CCD1b 1.44 0.85 0.59 1.04 2.15 2.06 1.13 0.60 0.53 0.74 1.25 1.68 2.63 1.02 0.39 3.47 3.35 0.97
9–LOX
HPL – – – – – – – – – – – – 2.19 5.98 2.73 1.66 3.64 2.19
LOXA – – – – – – – – – – – – 3.67 3.81 1.04 0.75 1.08 1.44
DES – – – – – – – – – – – – 3.05 9.47 3.10 3.34 5.73 1.72
AOS 3 – – – – – – – – – – – – 1.12 7.18 6.43 0.03 0.17 5.19
13-LOX
LOXD 3.08 4.39 1.43 1.65 2.33 1.41 0.26 0.30 1.16 0.28 0.42 1.47 14.8 7.60 0.51 14.4 10.4 0.72
AOS 1 – – – – – – – – – – – – 4.49 3.68 0.82 2.85 2.81 0.98
JAZ 2 1.78 9.99 5.62 3.81 5.72 1.50 0.44 0.33 0.75 0.16 0.28 1.71 10.2 17.3 1.70 12.9 4.46 0.34
JMT 0.98 1.53 1.56 0.63 2.16 3.44 0.27 0.76 2.85 0.23 1.12 4.87 0.59 1.81 3.09 0.86 0.62 0.72
Wound response
PS 0.0009 0.0012 1.33 0.001 0.003 3.68 0.0017 0.0016 0.93 0.0013 0.0010 0.79 0.0023 0.0047 2.01 0.0028 0.0011 0.39
RBOH1 0.30 0.07 0.24 0.09 0.23 2.63 0.33 0.14 0.42 0.22 0.16 0.73 0.26 0.13 0.51 0.23 0.13 0.55
LHA1 3.11 1.11 0.36 1.51 2.24 1.48 1.35 1.19 0.88 1.25 0.82 0.65 2.40 2.35 0.98 1.88 2.74 1.46
PLA2 – – – – – – – – – – – – 0.57 0.49 0.85 0.54 0.63 1.18
PINII 0.41 0.74 1.79 0.45 3.13 6.95 0.013 0.005 0.40 0.0025 0.0015 0.62 0.68 0.20 0.30 0.11 0.05 0.41
AroGP3 0.21 0.04 0.22 0.08 0.12 1.51 0.61 0.30 0.49 0.72 0.45 0.63 1.27 0.27 0.21 0.53 0.49 0.92
PPO – – – – – – – – – – – – 4.87 9.58 1.97 0.82 1.73 2.11
CYP2 25.8 19.3 0.75 14.5 20.6 1.42 39.2 30.6 0.78 51.4 43.2 0.84 72.4 122 1.69 19.2 52.0 2.70
SCP 0.21 0.07 0.35 0.23 0.30 1.28 0.72 0.35 0.49 0.69 0.38 0.55 0.35 0.25 0.72 0.18 0.33 1.81
Ethylene biosynthesis
ACS2 0.03 0.01 0.47 0.05 0.15 2.87 0.03 0.01 0.40 0.02 0.03 1.46 0.47 0.11 0.23 0.24 0.11 0.44
ACS6 – – – – – – – – – – – – 25.5 16.8 0.66 7.26 11.7 1.61
ACO4 3.13 2.22 0.71 3.24 5.70 1.76 2.53 3.20 1.27 3.54 1.77 0.50 47.3 40.2 0.85 12.6 25.8 2.04
Ethylene signaling
CTR4 – – – – – – – – – – – – 2.21 3.35 1.52 1.94 1.64 0.85
EIN2 1.00 0.30 0.30 0.23 0.34 1.49 1.12 0.73 0.65 1.07 0.12 0.11 8.32 6.49 0.78 4.70 14.07 2.99
EIN3 0.27 0.17 0.64 0.20 0.58 2.86 0.25 0.25 0.99 0.21 0.09 0.45 1.45 1.29 0.89 1.50 5.80 3.86
ERF1b – – – – – – – – – – – – 0.20 2.40 12.03 0.09 0.97 11.15
Salicylic acid signaling
PR1 0.105 0.011 0.10 0.03 0.05 2.01 0.11 0.04 0.31 0.07 0.08 1.23 – – – – – –
SSI2 0.008 0.007 0.94 0.003 0.015 5.05 0.0014 0.0013 0.88 0.0023 0.0004 0.15 – – – – – –
SAMT 0.006 0.002 0.34 0.01 0.04 3.81 0.14 0.10 0.68 0.31 0.42 1.35 – – – – – –
WRKY60 0.81 0.69 0.85 0.37 0.65 1.78 0.84 0.82 0.98 1.12 0.42 0.38 – – – – – –
Phenylpropanoid/phenolic compounds biosynthesis
PAL3 6.09 2.17 0.36 2.84 9.20 3.24 3.93 2.28 0.58 3.86 3.88 1.01 55.9 27.4 0.49 31.2 22.4 0.72
PAL 4 61.3 72.6 1.18 32 88 2.74 66.1 42.4 0.64 33.4 30.4 0.91 795 341 0.43 407 367 0.90
PAL 5 – – – – – – – – – – – – 39.1 54.6 1.40 50.1 54.5 1.09
FLS – – – – – – – – – – – – 0.52 1.27 2.46 0.46 1.04 2.26
Isoprenoid biosynthesis/volatiles
DXS–2 – – – – – – – – – – – – 0.14 0.78 5.51 0.09 0.12 1.37
FPS1 1.59 1.22 0.77 0.86 2.72 3.15 4.47 2.43 0.54 3.19 3.69 1.16 4.62 2.21 0.48 1.81 1.47 0.81
HMGR 1 – – – – – – – – – – – – 4.56 2.94 0.64 4.34 4.53 1.04
BEAT – – – – – – – – – – – – 0.30 2.92 9.87 0.06 0.07 1.16
Glycoalkaloid biosynthesis
GAME 1 0.32 0.15 0.49 0.23 0.46 1.97 2.01 1.19 0.59 1.16 0.83 0.72 1.19 0.88 0.74 0.68 0.91 1.35
CAS 1 0.40 0.16 0.40 0.23 0.57 2.55 0.56 0.28 0.50 0.33 0.34 1.05 0.42 0.48 1.14 0.34 0.49 1.41

Notes:

1

Gene expression levels were calculated using the 2−ΔCt equation, according to Livak & Schmittgen (2001).

2

C, represents expression levels quantified in roots of non-mycorrhizal control plants.

3

M, represents expression levels quantified in roots of mycorrhizal plants.

4

M/C, represents the gene expression ratio between roots of mycorrhizal and non-mycorrhizal plants.

5

The (–) symbol indicates that gene expression at these time points was not determined.

6

M/C values in bold text indicate a positive influence on gene expression by AMF colonization.

7

M/C values in red bold text indicate a negative influence on gene expression by AMF colonization.