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. Author manuscript; available in PMC: 2020 Apr 19.
Published in final edited form as: ACS Infect Dis. 2019 Sep 18;5(11):1952–1962. doi: 10.1021/acsinfecdis.9b00284

Figure 3. Time-of-addition experiments by quantify progeny virus yield in cell culture supernatant.

Figure 3.

RD cells were infected with EV-D68 US/MO/14–18947 at MOI = 0.01 at 0h time-point. At 1 hpi, inoculant virus was removed and washed with PBS buffer, progeny viruses in cell culture supernatant were harvested at 14 hpi and were quantified with plaque assay. Red arrow represents the period of time that drug (compound R856932 or pleconaril) was present in the cell culture. The concentrations used for R856932 and pleconaril were 25 μM and 12.5 μM, respectively. Asterisks indicate a statistically significant difference in comparison with the DMSO control (one-way analysis of variance analysis by Prism 5, *** P < 0.001). The value is the mean of two independent experiments ± S.D.