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. 2020 Apr 15;13:3129–3139. doi: 10.2147/OTT.S229997

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© 2020 Li et al.

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Knockdown of LMNB1 inhibits the proliferation of LUAD cells. (A) The shLMNB1 lentiviral-based vector was transfected into A-549 and PC-9 cell lines. The protein expression levels of LMNB1 was verified by Western blot after transfection. The histogram is a semi-quantitative analysis of Western blot data (***P<0.001). (B) CCK8 assay was conducted to measure the growth in both A-549 and PC-9 cell lines after knockdown of LMNB1. The absorbance value was measured at 490 nm (**P<0.01, ***P<0.001). (C) Colony formation assay was conducted to measure the proliferation of A-549 and PC-9 cell lines after knockdown of LMNB1. Histogram was used to analyze the colony numbers (***P<0.001). (D) The expression levels of Ki67, pAKT and AKT was detected by Western blot after knockdown of LMNB1 in both A-549 and PC-9 cell lines. (E) The expression level of LMNB1, Ki67, pAKT and AKT were determined by using semi-quantitative analysis of Western blot data in both A-549 and PC-9 cell lines. (***P<0.001, ns means no statistical significance).