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. 2020 Apr 17;3(5):e202000707. doi: 10.26508/lsa.202000707

Figure S3. Characterization of ventricle-like structures in human cerebral organoids (hCOs).

Figure S3.

(A) Localization of SOX2 (radial glia) and TUJ1 (neurons) in hCOs at 4, 8, and 12 wk of age, co-stained with DAPI, was visualized by immunofluorescence microscopy. SOX2+ radial glial cells line the apical space of the ventricular zone adjacent to the hollow ventricle structure. Triangles denote TUJ1+ radial glial processes oriented perpendicular in the ventricular zone (VZ) and arrows denote TUJ1+ neurons with parallel processes in the cortical plate (CPL). The dashed line denotes layering present in hCOs. Scale bar = 50 μm. (B) Localization of SOX2 (radial glia), CTIP2, and SATB2 (cortical layer markers) in hCOs at 4, 8, 12, 18, and 24 wk of age, co-stained with DAPI, was visualized by immunofluorescence microscopy. Scale bar = 500 μm for whole COs. CP, cortical plate; SVZ, sub-ventricular zone; VZ, ventricular zone.