Figure 2.

Direct imaging and tracking indentation-induced muscle deformation as a function of tissue location and the indentation depth. (a) A muscle sample was compressed up to 0.9 mm with an indenter in a cross-fiber fashion while the entire depth of the muscle was imaged with a confocal microscope. (b) Muscle samples were labeled with Hoechst nuclear staining (blue), and the images below show the compaction of the muscle tissue prior to (upper) and post indentation (lower). A total of 4679 points (green) at various depths from muscle surface (red, dotted line) underneath the indenter (light blue) were selected as representative tissue features and their locations in the time series of confocal images were tracked using MATLAB.