FIG 7.
Impact of VasH on T6SS-mediated activities. (A) Images of blue fluorescence from spots of bacterial growth resulting from mixtures of ES114 cells expressing CFP and the indicated FQ-A001-derived strain. Strains used in this experiment were FQ-A001 (WT), NPW58 (Δhcp Δhcp1), and KRG005 (ΔvasH). The scale bar indicates 5 mm. (B) Cellular abundance of ES114 and FQ-A001 mixtures at 0 h and 5 h postincubation. The y axis shows the ratio of erythromycin-resistant (Ermr) CFU to total CFU per mixture (spot). A two-way ANOVA revealed significant differences between log-transformed group means as a function of time. Sidak’s post hoc test was performed to statistically compare the means, with P values adjusted for multiple comparisons. Comparisons between groups with different letters have significantly different medians (P < 0.0001), while comparisons between groups with the same letter do not have significantly different medians (P > 0.05). Results shown are from a representative trial of an experiment performed twice.