TABLE 1.
Strains and plasmids
| Strain or plasmid | Genotype | Source or reference |
|---|---|---|
| Strains | ||
| ES114 | Wild-type V. fischeri | 47 |
| FQ-A001 | T6SS+ wild-type V. fischeri | 21 |
| KRG005 | FQ-A001 ΔvasH | This study |
| KRG007 | FQ-A001 ΔrpoN | This study |
| NPW58 | FQ-A001 Δhcp Δhcp1 | 14 |
| TIM313 | ES114 Tn7::erm | 46 |
| KRG003 | ES114 ΔrpoN | This study |
| EVS102 | ES114 ΔluxCDABEG | 48 |
| Plasmids | ||
| pSCV38 | pVSV105 PtetA-yfp PtetA-mCherry | 49 |
| pYS112 | pVSV105 PtetA-cfp PtetA-mCherry | 21 |
| pKRG001 | Phcp-gfp | This study |
| pKRG024 | Ptrc-vasH | This study |
| pNPW015 | Phcp1-gfp | This study |
| pVSV105 | R6Kori ori(pES213) RP4 oriT cat | 50 |
| pKV494 | ErmR flanked by FRT sites | 45 |
| pKV496 | FLPase | 45 |
| pTM214 | pVSV105 Ptrc-mCherry | 51 |
| pTM267 | pVSV105 PtetA-mCherry kan::gfp | 46 |