TABLE 2.
Primers
| Primer name | Sequence (5′→3′) |
|---|---|
| FQ_rpoN Del Up F | CCTCAAGAAGCTTCTATTTTTAGA |
| FQ_rpoN Del Up R | TAGGCGGCCGCACTAAGTATGGTATTTAGCGATACCTTTTGTACAT |
| FQ_rpoN Del Down F | GGATAGGCCTAGAAGGCCATGGTTAATTAAAAGGAAGTGTTATGCA |
| FQ_rpoN Del Down R | GATAGCTATCCCATTACCTATACC |
| FQ rpoN check ext F | CGCTCAAAAATGGGGATAGGCTATC |
| FQ rpoN check int F | CAATTTAAGTTGTAATGTACAAAAG |
| FQ rpoN check ext R | GCTTACATTGTTCATCAGGAACGAG |
| FQ_vasH Check int u2 | ATTTCTTAATAAAAAACCGCCTTCG |
| FQ_vasH Check int l2 | TACTCAAATCGTGATTTCAATTGATG |
| FQ_vasH Check ext l2 | GGATGAAGTTGAAAAAGCAGATCCA |
| EYFP SalI u1 | CGCGAGCTCATGCCAACTCCAGCATATATGTCAA |
| EYFP XmnI l1 | ATGGTACCTTATGCTTCGCGTGGTGTACGCCAGTC |
| cL1 | CCATACTTAGTGCGGCCGCCTA |
| cL2 | CCATGGCCTTCTAGGCCTATCC |
| FQ_vasH Del Up F | GTATTCGATAAAGGCACATTAAATG |
| FQ_vasH Del Up R | TAGGCGGCCGCACTAAGTATGGTTAGCTAAAGACATAACCTAGTGT |
| FQ_vasH Del Down F | GGATAGGCCTAGAAGGCCATGGATGAAACCATACTTACTATTATTAC |
| FQ_vasH Del Down R | ATAGAAAAAGAGTGAATTACATGAA |
| FQAvasH_iptg-SacI-u1 | ATTGAGCTCTTAAAGTATCTGTTCAGATGAAATATTATG |
| FQAvasH_iptg-KpnI-l1 | GTGGTACCCTCAAATCGTGATTTCAATTGATGA |
| FQ_Phcp1-gfp–u1 | ATTCCCGGGCTTGGAGCCTTATAATTTTAGAACA |
| FQ_Phcp1-gfp–l1 | CGTCTAGAGGCTTTATTCCTTAATATTAAATTCTATTG |
| FQ_Phcp-gfp_XmaI-u1 | ATTCCCGGGATTTATCATAGTTAGTTTCAATCA |
| FQ_Phcp-gfp_XbaI-l1 | CGTCTAGAGCTATTATCCTTTCATTAATAATTATT |
| ES_rpoN Del Up F | CCTCAAGAAGCTTCTATTTTTAGAA |
| ES_rpoN Del Up R | TAGGCGGCCGCACTAAGTATGGTATTTAGCGATACCTTTTGTACATT |
| ES_rpoN Del Down F | GGATAGGCCTAGAAGGCCATGGTTAATGAAAAGGAAGTGTTATGCAA |
| ES_rpoN Del Down R | GATAGCTATCCCATTACCTATACCA |
| ES_rpoN_Check inner F | GTTGTAATGTACAAAAGGTATCGCT |
| ES_rpoN_Check inner R | TTGCATAACACTTCCTTTTCATTAA |
| ES_rpoN_Check outer F | TTCGATTACTATTGATGGTGTCGAC |
| ES_rpoN_Check outer R | GGACGATTATCAATAGCATCAAATT |