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Deletion of MpfA attenuates Co(II) and Mn(II) toxicity. A growth assay was carried out in LB medium to evaluate sensitivity of the wild type and ΔyhdQ and mpfA null mutants to Mn(II) (A) and Co(II) (B) intoxication. The concentrations of MnCl2 and CoCl2 used were 700 μM and 300 μM, respectively. Experiments were performed with three biological replicates. Representative growth curves are shown.
