TABLE 1.
Isolated cobalt- and manganese-resistant suppressorsa
| Metal | Background | Selection conditions | No. of hits (no. of unique hits) | Target | Function |
|---|---|---|---|---|---|
| Manganese | mntR mntH | 200 μM MnCl2 | 5 (5) | mpfA | Putative magnesium exporter |
| Manganese | mntR mntH | 200 μM MnCl2 | 2 (2) | yhdQ | MerR family regulator |
| Manganese | mntR mntH | 200 μM MnCl2 | 1 | yeaD | Unknown |
| Manganese | mntR mntH | 200 μM MnCl2 | 1 | 5′ UTRb MgtE riboswitch | Regulation of magnesium uptake |
| Cobalt | czcD | 400 μM CoCl2 | 16 (12) | yhdQ-mpfA | YhdQ, MerR family regulator |
| MpfA, putative magnesium exporter |
a
15 μg ml−1 kanamycin was used to select the specific transposon insertion suppressors.
b
UTR, untranscribed region.