TABLE 3.
Isolated cobalt- and manganese-resistant suppressors under iron-limited conditions
| Metal | Background | Selection conditions | No. of hits (no. of unique hits) | Target | Function |
|---|---|---|---|---|---|
| Manganese | mntR Pspac-frvA | 75 μM MnCl2, 100 μM IPTG | 16 (11) | mntH | Manganese importer |
| Manganese | mntR mntH Pspac-frvA | 200 μM MnCl2, 100 μM IPTG | 1 | 5′ UTRa MgtE riboswitch | Regulation of magnesium uptake |
| Manganese | mntR mntH Pspac-frvA | 200 μM MnCl2, 100 μM IPTG | 1 | yhdQ | MerR family regulator |
| Manganese | mntR mntH Pspac-frvA | 200 μM MnCl2, 100 μM IPTG | 18 | Pspac-frvA | Ferrous iron efflux transporter |
| Cobalt | czcD Pspac-frvA | 400 μM CoCl2, 100 μM IPTG | 1 | putP | High-affinity proline permease |
| Cobalt | czcD Pspac-frvA | 400 μM CoCl2, 100 μM IPTG | 1 | ymfF | Putative multidrug-efflux transporter |
| Cobalt | czcD Pspac-frvA | 400 μM CoCl2, 100 μM IPTG | 1 | queF | Queosine biosynthesis |
| Cobalt | czcD Pspac-frvA | 100 μM CoCl2, 100 μM IPTG | 1 | mpfA | Putative magnesium exporter |
| Cobalt | czcD Pspac-frvA | 100 μM CoCl2, 100 μM IPTG | 1 | ybaF | Putative cobalt ABC transporter permease |
| Cobalt | czcD Pspac-frvA | 400 μM CoCl2, 1 mM IPTG | 12 | Pspac-frvA | Ferrous iron efflux transporter |
| Cobalt | czcD Pspac-frvA | 400 μM CoCl2, 1 mM IPTG | 1 | mpfA | Putative magnesium exporter |
| Cobalt | czcD Pspac-frvA | 400 μM CoCl2, 1 mM IPTG | 1 | yvrB | Putative vitamin B12 permease |
| Cobalt | czcD Pspac-frvA | 400 μM CoCl2, 1 mM IPTG | 1 | fur | Ferric uptake regulator |
| Cobalt | czcD Pspac-frvA | 400 μM CoCl2, 1 mM IPTG | 1 | ybcF | Putative carbonic anhydrase |
a
UTR, untranscribed region.