TABLE 3.
NADH oxidase activity of isolated membranes from E. faecalis strains grown in TSBG with or without hemin supplementation
| Strain | Gene inactivated | Hemin (2 μM) added to growth medium | NADH oxidase activity (%)a |
|---|---|---|---|
| OG1RF | Yes | 100 ± 6 | |
| No | <8 | ||
| ndh3::Tn | ndh3 | Yes | 75 ± 11 |
| No | <8 | ||
| pplA::Tn | pplA | Yes | 126 ± 6 |
| No | <8 | ||
| WY84 | menB | Yes | <8 |
| No | <8 | ||
| LFR1 | Intergenic ndh3-eetA | Yes | 46 ± 1 |
| No | <8 | ||
| LFR9 | eetA | Yes | 68 ± 2 |
| No | <8 | ||
| LFR16 | ndh3 | Yes | 43 ± 2 |
| No | <8 |
a
One hundred percent activity was 0.53 nmol NADH oxidized per minute and mg of protein.