TABLE 2.
The efficiencies of the promoter activities at each positiona
| Base | Relative CAT activity |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| –35 box at position: |
–10 box at position: |
|||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | 0.42 IJ | 0.38 JK | 0.34 KL | 0.77 G | 0.58 H | 0.77 G | <0.1 O | 1 D | 0.46 I | 1 D | 1 D | <0.1 O |
| C | 0.39 J | 0.38 JK | 0.32 L | 2.03 B | 0.88 E | 1 D | <0.1 O | <0.1 O | 1 D | 0.17 M | 0.54 H | <0.1 O |
| G | <0.1 O | 0.46 I | 1 D | 0.32 L | 1 D | 0.40 J | <0.1 O | <0.1 O | 0.77 G | 0.82 F | 0.20 M | <0.1 O |
| T | 1 D | 1 D | 0.53 H | 1 D | 0.78F G | 1.42 C | 1 D | <0.1 O | 2.98 A | 0.89 E | 0.12 N | 1 D |
| Consensus | T | T | G | T | G | C | T | A | C | A | A | T |
| Optimal | T | T | G | C | g | t | T | A | T | a | A | T |
a
The control strain had plasmid pKO403-TPCTcon (Fig. S3). The others had 1-bp mutations in the promoter regions of their plasmids. The position numbers are related to Fig. 1A. The results shown are relative to the value for the control. “Optimal” shows the highest bases in each position. Uppercase letters indicate that the highest bases showed 1.5 times as much activity as the second-highest bases. Boldface indicates the strongest activity in each position. Different letters indicate statistically distinguishable groups (P < 0.01; Tukey’s multiple-comparison test).