FIG 4.

RmlA and RsbU influence biofilm formation by L. monocytogenes EGDe. (A) The biomasses of WT₁₀₃₁, WT₁₀₃₀, WT₁₀₃₁ Δlmo0184 (LSW1024), WT₁₀₃₁ ΔrsbU (LSW1028), WT₁₀₃₁ ΔrmlA (LSW1040), and WT₁₀₃₁ ΔrmlA ΔrsbU (LSW1051) strains that were adherent to the substratum were quantified. The samples were incubated at 30°C for the time points indicated. The values presented for WT₁₀₃₁ and WT₁₀₃₀ are reproduced from Fig. 3. The means from ≥4 experiments are presented for the remaining strains. The error bars are the standard errors of the means. The data were analyzed by one-way ANOVA, comparing with WT1031. *, P ≤ 0.05; **, P ≤ 0.01. The biomasses adherent to the substratum were imaged using scanning electron microscopy for WT₁₀₃₁ (B), WT₁₀₃₀ (C), WT₁₀₃₁ Δlmo0184 (D), WT₁₀₃₁ ΔrsbU (E), WT₁₀₃₁ ΔrmlA (F), and WT₁₀₃₁ ΔrmlA ΔrsbU (G). The representative images shown were taken at the midpoint of the peg after 48 h of incubation.