FIG 5.

SigB influences biofilm formation by L. monocytogenes EGDe. (A) The biomasses of WT₁₀₃₁, WT₁₀₃₁ ΔrsbU (LSW1028), and WT₁₀₃₁ ΔsigB (LSW1026) strains that were adherent to the substratum were quantified. The samples were incubated at 30°C for the time points indicated. The values presented for WT₁₀₃₁ and WT₁₀₃₁ ΔrsbU are reproduced from Fig. 3 and 4. The means from ≥4 experiments are presented for the WT₁₀₃₁ ΔsigB strain. The error bars are the standard errors of the means. The data were analyzed by one-way ANOVA comparing with WT1031. *, P ≤ 0.05; **, P ≤ 0.01. (B) The biomass adherent to the substratum was imaged using scanning electron microscopy for WT₁₀₃₁ ΔsigB. The representative image shown was taken at the midpoint of the peg after 48 h of incubation.