FIG 1.

EcGTPase-CcCTC can cause bulging and lysis similar to that with CcΔCTL. (A) Phase-contrast images of morphology and merged epifluorescence images showing HADA incorporation (yellow) overlaid on phase-contrast images (blue) corresponding to cells depleted of FtsZ and simultaneously induced for xylose-dependent production of C. crescentus FtsZ (cyan), E. coli FtsZ (magenta), CTL truncations, or their chimeric variants. Phase-contrast images were acquired after 5 h of induction of FtsZ variant. HADA fluorescence images were acquired after 4.5 h of induction of FtsZ variant. Scale bar, 2 μm. (B) Growth characteristics of strains shown in panel A represented as absorbance at OD₆₀₀ over time. Shaded regions represent standard deviations of three technical replicates at each point. Strains are as indicated, and all are depleted of native CcFtsZ.