FIG 7.
FtsA overproduction interferes with Z-ring assembly and causes mNG-ΔCTL to form long helical structures in vivo. Shown are orthogonal views of 3D projections of image stacks acquired by 3D-SIM visualization of Z rings in cells induced with xylose to drive expression of empty vector and mNG-FtsZ (A), ftsA and mNG-FtsZ (B), empty vector and mNG-ΔCTL (C), or ftsA and mNG-ΔCTL (D) from the PxylX promoter for the indicated amounts of time. Compasses in the lower left of images indicate the orientation of long (green) and short (blue) axes of cells. Scale bar, 1 μm.