Collection	Collection is easiest from the rumen of a fistulated adult cow. If a fistulated cow is unavailable, fluid can be collected through a weighted orogastric tube. Alternatively, fluid can be collected from any normal ruminant at slaughter.
Handling	Rumen contents collected from a fistulated cow or at slaughter can be strained through gauze or cheesecloth to separate the fluid from the fibrous contents. Fluid collected through a weighted tube should be ready for storage.
Storage	Rumen fluid should ideally be administered immediately. However, it can be stored for 24 to 48 hours. The surface of the fluid should be covered with a layer of mineral oil to maintain an anaerobic environment and stored at refrigerator temperature. CAUTION: Do not store rumen fluid in a closed container because it may explode.