Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Dec 2;13(23-24):3442–3456. doi: 10.1002/pmic.201300001

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim

This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.

PMC Copyright notice

Expression of JEV NS5 protein in TE671 human medulloblastoma cells. Cells transfected with pCR3.1‐Flag (control vector; left) or pCR3.1‐JEV NS5‐Flag (right) were selected by 2‐wk incubation with G418. Expression of Flag‐epitope tagged protein was examined by fluorescence microscopy (A). Also, Western blot to detect expressed NS5 was conducted via anti‐Flag antibody (B). Lysates from cells transfected with pCR3.1‐Flag (lane 1) or pCR3.1‐JEV NS5‐Flag (lane 2) were analyzed by 10% SDS‐PAGE prior to blotting, resulting blots probed with anti‐Flag tag antibody.