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. 2013 Dec 2;13(23-24):3442–3456. doi: 10.1002/pmic.201300001

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© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim

This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.

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Functional characterization of calreticulin with siRNA‐mediated gene silencing. (A) To detect intracellular Ca²⁺, cells transfected with control or calreticulin siRNA were harvested 4 h posttreatment with or without IFNβ, stained with FLUO3/AM and analyzed by flow cytometry. To analyze subcellular localization of STAT1 (B, C) and NFAT‐1 (D, E), vector control (B, D) and NS5‐expressing cells (C, E) transfected with control or calreticulin siRNA were tested by immunfluorescent staining with anti‐STAT1 or anti‐NFAT‐1 antibodies.