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Functional analysis of calcineurin with siRNA‐mediated gene silencing. To analyze subcellular localization of STAT1, vector control (A) and NS5‐expresing cells (B) transfected with control or calreticulin siRNA were tested using immunfluorescent staining with anti‐STAT1 antibodies. Relative mRNA levels of PKR (C) and IL‐4 (D) were measured by quantitative PCR and normalized by GAPDH mRNA, presented as relative ratio.
