Table 1.
Up‐ and down‐regulated proteins in NS5‐expressing cells compared to vector control cells
| Spot ID | PANTHER gene ID | Protein identification | MASCOT score | MW/pI | Numbers of peptides identified | Sequence coverage (%) | Molecular function | Biological process | Fold change (±SD) |
|---|---|---|---|---|---|---|---|---|---|
| Upregulated proteins in NS5‐expressing cells compared to vector control cells | |||||||||
| 1 | 3615 | Inosine‐5′‐monophosphate dehydrogenase 2 (IMPDH 2) | 451 | 55.8/6.44 | 11 | 35 | Oxidoreductase | Purine metabolism | 4.16 ± 0.27↑ |
| 4 | 3329 | 60 kDa HSP (Hsp60) | 1145 | 61/5.7 | 15 | 52 | Protein folding, protein complex assembly | 1.71 ± 0.01↑ | |
| 5 | 3184 | Heterogeneous nuclear ribonucleoprotein D0 (hnRNP D0) | 96 | 38.4/7.62 | 2 | 6 | RNA splicing factor, transesterification, structural constituent of ribosome poly(A) RNA binding | mRNA splicing | 3.42 ± 0.05↑ |
| 6 | 3315 | HSP beta‐1 (HSPB1, Hsp27–1) | 458 | 22.8/5.98 | 5 | 66 | Structural molecule | Protein folding, stress response | 1.60 ± 0.32↑ |
| 8 | 5052 | Peroxiredoxin‐1 (PRDX1) | 192 | 22.1/8.27 | 4 | 13 | Oxidoreductase peroxidase | Antioxidation and free radical removal | 3.28 ± 0.18↑ |
| 9 | 10963 | Stress‐induced‐phosphoprotein 1 (STIP1) | 70 | 62.6/6.40 | 6 | 11 | Stress response | 2.08 ± 0.11↑ | |
| 12 | 5478 | Cyclophilin A (CYPA) | 119 | 18.09/7.82 | 11 | 68 | Isomerase | Protein folding, nuclear transport, immunity and defense | 1.72 ± 0.08↑ |
| Downregulated proteins in NS5‐expressing cells compared to vector control cells | |||||||||
| 2 | 26227 | d‐3‐phosphoglycerate dehydrogenase (3‐PGDH) | 424 | 56.6/6.29 | 13 | 33 | Oxidoreductase | Amino acid biosynthesis | 0.01 ± 0.04↓ |
| 3 | 3189 | Heterogeneous nuclear ribonucleoprotein H3 (HNRPH3) | 94 | 36.9/6.37 | 3 | 13 | Structural constituent of ribosome, nucleic acid binding | mRNA splicing | 0.12 ± 0.09↓ |
| 7 | 10576 | T‐complex protein 1 subunit beta (TCP‐1‐beta) | 129 | 57.5/6.01 | 7 | 20 | Chaperonin | 0.60 ± 0.06↓ | |
| 10 | 7295 | Thioredoxin (MTRX) | 86 | 11.7/4.82 | 2 | 19 | Oxidoreductase | Electron transport, sulfur redox metabolism, other intracellular signaling cascade, stress response, apoptosis, cell proliferation and differentiation | 0.27 ± 0.11↓ |
| 11 | 5245 | Prohibitin (PHB) | 72 | 29.8/5.57 | 7 | 37 | DNA replication, cell cycle control, cell proliferation and differentiation | 0.12 ± 0.07↓ | |
| 13 | 811 | Calreticulin precursor (CALR) | 101 | 63.89/4.3 | 8 | 24 | Calcium ion binding | Protein folding | 0.51 ± 0.12↓ |
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