Table 2.
Viruses Detected by Real‐Time PCR in Direct Immunofluorescence (DIF) Positive and Negative Children
|
Virus(es) detected by real‐time PCR 1 |
No. patients (%) |
|---|---|
|
Direct immunofluorescence positive patients (n = 19) 2, 3 | |
| Respiratory syncytial virus | 13 (68) |
| Respiratory syncytial virus + rhinovirus | 5 (26) |
| Respiratory syncytial virus + rhinovirus + coronavirus | 1 (5) |
|
Total no. of real‐time PCR positive patients 4 |
19 (100) |
|
Direct immunofluorescence negative patients (n = 19) 2 | |
| Respiratory syncytial virus | 4 (21) |
| Influenza viruses | 3 (16) |
| Rhinovirus | 1 (5) |
| Coronavirus | 1 (5) |
| Adenovirus | 1 (5) |
| Human metapneumovirus | 1 (5) |
| Human bocavirus | 1 (5) |
| Respiratory syncytial virus + rhinovirus | 1 (5) |
| Respiratory syncytial virus + human bocavirus | 1 (5) |
| Respiratory syncytial virus + human metapneumovirus | 1 (5) |
| Coronavirus + parainfluenza virus | 1 (5) |
|
Total no. of real‐time PCR positive patients 5 |
16 (84) |
PCR was performed for respiratory syncytial virus, influenza viruses, parainfluenza viruses, adenoviruses, rhinoviruses, coronaviruses, human metapneumovirus, Mycoplasma pneumoniae, and human bocavirus.
Direct immunofluorescence (DIF) was performed for respiratory syncytial virus, influenza viruses, parainfluenza viruses, and adenoviruses.
The DIF positive patients were all positive for respiratory syncytial virus.
In the DIF positive group real‐time PCR identified a total of 26 viruses (The 19 viruses identified by DIF plus 7 additional viruses).
In the DIF negative group real‐time PCR identified a total of 20 viruses.