Table I.
Description of nSS Trx Mutants
| WT Trx sequencea | CX1X2C mutantb | Position of CX1X2C in the helixc |
|---|---|---|
| NIDQ (59–62) | CIPC | N‐Cap‐3 |
| NIDQ (59–62) | CIDC | N‐Cap‐3 |
| NIDQ (59–62) | CGPC | N‐Cap‐3 |
| SKGQ (95–98) | CKGC | N‐Cap‐3 |
| SKGQ (95–98) | CGPC | N‐Cap‐3 |
| IDQN (60–63) | CDQC | 1–4 |
| KGQL (96–99) | CGQC | 1–4 |
| FLDA (102–105) | CLDC | 7–10 |
a
Sequence before mutation in nSS Trx. The numbers in parentheses are the residue numbers of the two residues mutated to cysteines.
b
The identities of residues X1,X2 between the introduced cysteines are shown.
c
N‐Cap is the first residue of the relevant helix as defined in PDB records. The residue immediately C‐terminal to N‐Cap in the helix is residue 1.