Table 2.
Lowest titres of dengue virus serotypes detected by dengue nonstructural 1 antigen (NS1 Ag) detection assays.
| Dengue Virus (DENV) | Dengue NS1 Ag Rapid Assays | Dengue NS1 Ag ELISAs | |||
|---|---|---|---|---|---|
| SD BIOLINE Dengue NS1 Ag Rapid Test | Panbio Dengue Early Rapid | Bio-Rad Dengue NS1 Ag STRIP | SD Dengue NS1 Ag ELISA | Panbio ELISA | |
| DENV-1 | 3.35 ± 0.09 (7.21 ± 0.24) |
4.22 ± 0.14 (8.07 ± 0.07) |
4.22 ± 0.14 (8.07 ± 0.07) |
0 (5.41 ± 3.13) |
3.25 ± 0.02 (7.00 ± 0.12) |
| DENV-2 | 3.59 ± 0.04 (7.70 ± 0.14) |
4.08 ± 0.20 (8.10 ± 0.06) |
3.59 ± 0.04 (7.70 ± 0.14) |
0.57 ± 0.98 (2.03 ± 3.51) |
3.12 ± 0.07 (7.10 ± 0.21) |
| DENV-3 | 3.07 ± 0.18 (7.58 ± 0.10) |
3.39 ± 0.04 (8.15 ± 0.04) |
3.07 ± 0.18 (7.58 ± 0.10) |
0.57 ± 0.98 (5.40 ± 0.29) |
2.20 ± 0.17 (6.64 ± 0.22) |
| DENV-4 | 3.75 ± 0.18 (9.15 ± 0.13) |
4.22 ± 0.10 (9.83 ± 0.26) |
2.94 ± 0.10 (8.83 ± 0.12) |
0 (6.66 ± 0.15) |
2.43 ± 0.23 (8.17 ± 0.02) |
DENV cultures were harvested every 6 h postinfection (h.p.i.) until 60 h.p.i. and were titrated in triplicate. RNA copy numbers were also quantitated in the same triplicate samples by using a probe-based quantitative real-time PCR (qRT-PCR) assay [18]. Each supernatant was tested with DENV NS1 Ag detection assays according to the manufacturers’ instructions. The testing was done in two independent time-course experiments. The titres shown are mean values, expressed in log pfu/mL ± standard deviation and log RNA copies/mL ± standard deviation (in brackets). pfu: plaque-forming unit.