Table 5.
Effect of ascorbic acid and β-1,3-glucan addition on the free amino acids of tiger grouper during simulated transport (mg/100 g).
| Transport | Samples | Free Amino Acids | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Asp * | Thr # | Ser # | Glu * | Gly # | Ala # | Val | Met | Ile | ||
| Before transport | CK | 1.84 ± 0.25a | 7.12 ± 0.25c | 3.98 ± 0.17b | 4.06 ± 0.22b | 49.40 ± 0.49d | 38.22 ± 0.71a | 5.45 ± 0.31a | 2.96 ± 0.26a | 4.73 ± 0.34a |
| A | 1.76 ± 0.01a | 11.44 ± 0.06b | 4.87 ± 0.25a | 4.89 ± 0.71a | 86.09 ± 0.91a | 34.33 ± 0.59b | 4.30 ± 0.51b | 2.07 ± 0.21a | 3.01 ± 0.22b | |
| A-G1 | 1.65 ± 0.26a | 10.93 ± 0.18b | 3.77 ± 0.17b | 5.03 ± 0.14a | 75.24 ± 0.69b | 31.29 ± 0.06c | 5.98 ± 0.08a | 2.56 ± 0.18a | 3.97 ± 0.62a | |
| A-G2 | 1.79 ± 0.32a | 12.14 ± 0.21a | 4.05 ± 0.38b | 4.42 ± 0.61b | 71.25 ± 0.53b | 39.78 ± 0.71a | 5.09 ± 0.22a | 2.13 ± 0.11a | 4.17 ± 0.04a | |
| A-G3 | 1.84 ± 0.41a | 11.03 ± 0.01b | 4.97 ± 0.51a | 4.79 ± 0.25a | 66.42 ± 0.66c | 33.86 ± 0.81b | 4.97 ± 0.05a | 2.44 ± 0.01a | 3.57 ± 0.28b | |
| After transport | CK | 1.98 ± 0.03c | 5.87 ± 0.45c | 5.10 ± 0.05c | 8.57 ± 0.07a | 63.01 ± 0.21c | 42.56 ± 0.66b | 6.91 ± 0.06a | 3.32 ± 0.28a | 5.89 ± 0.41a |
| A | 3.37 ± 0.19a | 9.85 ± 0.06b | 7.62 ± 0.28a | 6.33 ± 0.21b | 124.35 ± 0.37a | 38.03 ± 0.08d | 5.13 ± 0.10b | 2.62 ± 0.01b | 2.50 ± 0.16c | |
| A-G1 | 2.38 ± 0.11b | 10.01 ± 0.32b | 7.99 ± 0.33a | 5.53 ± 0.03c | 75.44 ± 0.41b | 40.24 ± 0.19c | 6.02 ± 0.21a | 2.78 ± 0.06b | 3.11 ± 0.11b | |
| A-G2 | 1.93 ± 0.01c | 10.93 ± 0.22a | 6.46 ± 0.05b | 5.09 ± 0.10c | 78.03 ± 0.39b | 46.51 ± 0.73a | 5.67 ± 0.03b | 2.61 ± 0.01b | 3.55 ± 0.06b | |
| A-G3 | 1.70 ± 0.22c | 10.90 ± 0.06a | 5.48 ± 0.10c | 5.14 ± 0.19c | 74.29 ± 0.11b | 41.22 ± 0.37c | 5.13 ± 0.18b | 2.48 ± 0.12b | 3.71 ± 0.28b | |
| Recovery | CK | 1.82 ± 0.08a | 7.89 ± 0.18c | 4.77 ± 0.27a | 6.59 ± 0.47a | 58.98 ± 0.91c | 40.03 ± 0.57b | 6.84 ± 0.25a | 2.38 ± 0.18a | 4.84 ± 0.10a |
| A | 1.98 ± 0.10a | 11.19 ± 0.27a | 5.18 ± 0.27a | 5.94 ± 0.31b | 96.71 ± 0.43a | 35.28 ± 0.09d | 4.97 ± 0.33b | 2.46 ± 0.02a | 2.92 ± 0.16c | |
| A-G1 | 1.73 ± 0.02a | 10.52 ± 0.04b | 4.85 ± 0.03a | 4.96 ± 0.80c | 74.04 ± 0.09b | 37.90 ± 0.18c | 4.99 ± 0.09b | 2.10 ± 0.11a | 4.06 ± 0.71a | |
| A-G2 | 1.58 ± 0.16a | 11.88 ± 0.11a | 5.16 ± 0.18a | 4.83 ± 0.33c | 77.13 ± 0.36b | 41.64 ± 0.78a | 4.96 ± 0.18b | 1.92 ± 0.02a | 3.49 ± 0.39b | |
| A-G3 | 1.69 ± 0.22a | 11.85 ± 0.65a | 4.86 ± 0.20a | 4.31 ± 0.57c | 73.04 ± 0.74b | 37.49 ± 0.07c | 4.67 ± 0.21b | 2.23 ± 0.18a | 3.39 ± 0.27b | |
| Leu | Tyr | Phe | Lys | His | Arg | Pro# | Total | |||
| Before transport | CK | 7.52 ± 0.37a | 3.22 ± 0.02a | 2.88 ± 0.02a | 28.54 ± 0.54a | 3.86 ± 0.25a | 7.86 ± 0.71a | 6.27 ± 0.68a | 177.91 | |
| A | 7.73 ± 0.41a | 1.45 ± 0.01c | 1.57 ± 0.11c | 21.89 ± 0.48c | 3.55 ± 0.34b | 5.38 ± 0.42c | 5.49 ± 0.71b | 199.82 | ||
| A-G1 | 7.88 ± 0.68a | 1.73 ± 0.22b | 2.05 ± 0.18b | 25.33 ± 0.71b | 3.18 ± 0.33b | 6.93 ± 0.74bc | 5.93 ± 0.01a | 193.45 | ||
| A-G2 | 7.03 ± 0.31a | 1.98 ± 0.17b | 2.47 ± 0.17a | 26.09 ± 0.31b | 4.09 ± 0.06a | 6.41 ± 0.11b | 6.09 ± 0.39a | 198.98 | ||
| A-G3 | 7.97 ± 0.45a | 2.06 ± 0.28b | 1.99 ± 0.01c | 23.45 ± 0.78c | 3.74 ± 0.28a | 7.07 ± 0.02b | 5.77 ± 0.81ab | 185.94 | ||
| Aftertransport | CK | 7.83 ± 0.31a | 3.76 ± 0.28a | 3.46 ± 0.15a | 31.35 ± 0.59a | 4.30 ± 0.45a | 10.74 ± 0.33a | 8.18 ± 0.39a | 212.83 | |
| A | 7.76 ± 0.28a | 1.34 ± 0.01c | 2.28 ± 0.25b | 24.39 ± 0.63c | 3.63 ± 0.19b | 4.99 ± 0.41d | 6.07 ± 0.08c | 250.26 | ||
| A-G1 | 8.74 ± 0.41b | 1.43 ± 0.12c | 2.46 ± 0.06b | 29.39 ± 0.71b | 4.12 ± 0.06a | 6.63 ± 0.71c | 7.74 ± 0.73b | 214.01 | ||
| A-G2 | 7.01 ± 0.37a | 1.55 ± 0.08c | 2.49 ± 0.03b | 27.83 ± 0.39b | 4.23 ± 0.28a | 6.69 ± 0.07c | 6.41 ± 0.36c | 216.99 | ||
| A-G3 | 7.76 ± 0.63a | 2.38 ± 0.19b | 2.48 ± 0.11b | 24.87 ± 0.23c | 3.93 ± 0.09a | 7.98 ± 0.20b | 5.71 ± 0.51d | 205.16 | ||
| Recovery | CK | 6.82 ± 0.10b | 2.95 ± 0.01a | 1.79 ± 0.02b | 22.19 ± 0.33c | 3.60 ± 0.17a | 6.59 ± 0.38a | 5.19 ± 0.62c | 183.27 | |
| A | 6.98 ± 0.12b | 1.52 ± 0.02b | 1.98 ± 0.10ab | 22.75 ± 0.62c | 3.42 ± 0.31a | 5.21 ± 0.67b | 5.94 ± 0.17b | 214.43 | ||
| A-G1 | 7.65 ± 0.41a | 1.67 ± 0.13b | 2.30 ± 0.21a | 26.91 ± 0.15a | 2.75 ± 0.07b | 6.87 ± 0.37a | 6.79 ± 0.57a | 200.09 | ||
| A-G2 | 7.83 ± 0.57a | 1.86 ± 0.03b | 2.19 ± 0.09a | 24.50 ± 0.31b | 2.98 ± 0.35b | 6.61 ± 0.65a | 5.81 ± 0.08b | 204.37 | ||
| A-G3 | 7.23 ± 0.69a | 1.44 ± 0.02b | 2.26 ± 0.11a | 19.59 ± 0.09d | 3.64 ± 0.41a | 6.55 ± 0.52a | 4.94 ± 0.72c | 189.18 | ||
Note: Among different treatments transport groups, different small letters indicate the results of Duncan’s test at different transport time. The same letters mean no significant difference (p > 0.05), while different letters mean significant difference (p < 0.05). * represents umami amino acids; # represents sweet taste amino acids.