Table 2.
21 selected candidates for the siRNA experiments
| Selected candidate | Gene symbol | Mean ratioa) | MF annotationb) | Organelle annotationc) |
|---|---|---|---|---|
| Enriched proteins | ||||
| Stanniocalcin‐2 precursor | STC2 | 0.22 | M | |
| Solute carrier family 38 member 2 | SLC38A2 | 0.22 | MFU | PM |
| A‐kinase anchor protein 12 isoform 2 | AKAP12 | 0.55 | MFU | CP/CYT |
| 28 kDa heat‐ and acid‐stable phosphoprotein | PDAP1 | 0.57 | MFU | ? |
| Kinesin heavy chain | KIF5B | 0.59 | CYT | CP/CYT |
| Peroxiredoxin‐6 | PRDX6 | 0.64 | OX | CP/L |
| Depleted proteins | ||||
| Coronin‐1B | CORO1B | 1.46 | CYT | CP/CYT |
| Isoform β‐4C of Integrin β‐4 precursor | ITGB4 | 1.47 | MFU | PM |
| Engulfment adaptor PTB domain containing 1 | GULP1 | 1.50 | SM | CP |
| Isoform 2 of AP‐2 complex subunit β | AP2B1 | 1.54 | MT | CP/PM |
| Guanine nucleotide‐binding protein G(i), α‐1 subunit | GNAI1 | 1.55 | SRM | PM |
| Ferritin heavy chain | FTH1 | 1.57 | MIF | CP |
| Guanine nucleotide‐binding protein G(I)/G(S)/G(T) subunit b2 | GNB2 | 1.58 | SRM/HY | CP |
| Isoform 1 of Protein LAP2 | ERBB2IP | 1.60 | MIF | N/CP/PM |
| Stomatin | STOM | 1.62 | CYT | CYT/PM |
| Frizzled‐6 precursor | FZD6 | 1.69 | MFU | PM |
| Guanine nucleotide‐binding protein G | GNAI3 | 1.76 | SRM | G/CP/PM |
| Band 4,1‐like protein 2 | EPB41L2 | 1.87 | MFU | CYT/PM |
| Isoform 1 of Protein ITFG3 | ITFG3 | 1.90 | MFU | ? |
| G protein‐coupled receptor 56 isoform b | GPR56 | 2.19 | REC | PM |
| TBC domain containing protein 10B | TBC1D10B | 2.27 | SRM | ? |
a)
a)The mean ratio is the average ratio from three independent SILAC experiments.
b)
b)Molecular Function annotation: SM, signaling molecule; MFU, molecular function unclassified; CYT, cytoskeletal protein; OX, oxireductase; MT, membrane traffic; SRM, select regulatory molecule; MIF, miscellaneous function; HY, hydrolase; REC, receptor.
c)
c)?, unknown; PM, plasma membrane; CP, cytoplasm; CYT, cytoskeleton; L, lysosome; N, nucleus; G, Golgi apparatus.