Production of reactive oxygen species (ROS) in Gram-negative and Gram-positive bacteria. Production of ROS by susceptible and MDR strains of A. baumannii (ATCC 17978 and #11) (A), P. aeruginosa (PAO1 and Pa238) (B), E. coli (ATCC 25922 and Ecmcr1+) (C), S. aureus (Sa24 and USA300#1) (D), and E. faecalis (VS and VR) (E) in the presence of 0.25×, 0.5×, and 1× MIC of colloidal silver, 10 mg/L ampicillin, 10 and 64 mg/L ciprofloxacin, and 10 and 256 mg/L vancomycin for 24 h. CTL: control, bacteria without treatment. Data are represented as means ± standard errors of the means (SEM) from 12 replicates in three independent experiments. * p < 0.05: treatment vs. control. The MICs of colloidal silver against ATCC 17978, #11, PAO1, Pa238, ATCC 25922, Ecmcr1+, E. faecalis VS, E. farcalis VR, Sa24, and USA300#1 were 4, 4, 4, 8, 4, 8, 8, 8, 8, and 8 mg/L, respectively.