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Western blot test. Sera reactivity to OMPs. The OMP enriched extract from (a) E. coli O75 was resolved in a 12% SDS-PAGE stained with Coomassie blue. Proteins were transferred to a PVDF membrane and incubated with the sera of (b) post and (c) pre-treatment patients and (d) donors with no UTIs. OMPs between 30 and 37 kDa are inside the white square. Protein fractions marked with an asterisk were sent for sequencing and are listed in Table S2.
