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. 2020 Feb 10;9(2):72. doi: 10.3390/antibiotics9020072

Figure 1.

Figure 1

Effect of treatment of M. abscessus with clarithromycin and/or rifabutin on whiB7 and erm41 mRNA levels. M. abscessus ATCC19977 cultures were exposed for 30 min to clarithromycin (CLR), rifabutin (RFB) or a combination of both antibiotics and relative transcript levels were determined. Fold changes compared to untreated cultures are shown. CLR 5 µM: MIC of CLR. CLR 0.78 µM: subinhibitory CLR concentration used in the synergistic combination. CLR 0.78 µM + RFB 0.39 µM: synergistic, growth inhibitory combination. RFB 0.39 µM: subinhibitory RFB concentration used in the synergistic combination. Transcript levels were measured by qRT-PCR and normalized to rpsA as described [6]. Shown are mean values with standard deviations from two independent experiments. 0.78 µM CLR corresponds to 1 mg/L; 0.39 µM RFB corresponds to 0.46 mg/L.