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. 2020 Feb 17;9(2):31. doi: 10.3390/biology9020031

Figure 1.

Figure 1

Immunofluorescence staining of a hypoxic peri-arteriolar HSC niche in bone marrow (A,B) and a hypoxic peri-arteriolar GSC niche in a glioblastoma tumor (C,D). HSCs (A) and GSCs (C) express the stem cell marker CD133 and are localized around the tunica adventitia and the SMA-positive tunica media of an arteriolar wall (a) (A, C, yellow arrows). Receptor CXCR4 is expressed by HSCs (B) and GSCs (D) in SDF-1α-rich niches (B, D, yellow arrows). SDF-1α is expressed extracellularly and is also expressed by endothelial cells and smooth muscle cells in the tunica media of the arteriolar wall in both HSC niches and GSC niches (B, D). DAPI (blue) is used for nuclear counterstaining. Scale bar = 30 μm. Abbreviations: CXCR4, C-X-C receptor type 4; DAPI, 4′.6-diamidino-2-phenylindole; GSC, glioblastoma stem cell; HSC, hematopoietic stem cell; SDF-1α, stromal-derived factor-1α; SMA, smooth muscle actin.