Figure 2.

Toxicity of hydroethanolic root extract (HRE) and danazol. BJAB and DG-75 cells were incubated with increasing doses of the HRE or danazol for 3 days. (A) Metaphases from BJAB and DG75 cell lines stained with a Cy3 telomere probe (red) and FITC centromere probe (green) showing drastically short telomeres in BJAB cells. (B) Cell viability determined by manual cell counting after staining with trypan blue. Relative survival was calculated after in vitro exposure to the HRE or danazol. The data are representative of three independent experiments and expressed as the mean ratio ± standard error of the mean. The experiments were performed in triplicate. (C) Mitotic index variation after exposure to HRE and danazol for 3 days following a single dose of 1 µg/mL. Two slides were used for each condition.