Table 1.
Sequences of Oligonucleotide Primers and Probes Used for the Detection of hMPV and RSV in Patient Samples
| Target | Primer/probe name | Primer/probe sequence (5′–3′) |
|---|---|---|
| hMPVa | FF | CGTTTCTTACATGCCGACATCTG |
| hMPVa | FR | GCTCCCGTAGACCCCTATCAG |
| hMPVa | FP | (FAM)CCCTTTCTTCGCACCATCGCACGG(Eclipse) |
| hMPVb | 450F | CTTTGGACTTAATGACAGATG |
| hMPVb | 450R | GTCTTCCTGTGCTAACTTTG |
| hMPVb | GF | GAGAACATTCGRRCRATAGAYATG |
| hMPVb | GR | AGATAGACATTRACAGTGGATTCA |
| RSV | P1 | TGGGACACTCTTAATCAT |
| RSVc | P2 | TGATTCCAAGCTGAGGAT |
| RSVd | P3 | GTTGTATGGTGTGTTTC |
a
Nucleotide sequences for hMPV according to GenBank accession number DQ336144.
b
Nucleotide sequences for hMPV according to GenBank accession number AF371337.
c
Nucleotide sequences for RSV prototype strain A2 according to GenBank accession number M11486.
d
Nucleotide sequences for RSV prototype strain B (strain 18537) according to GenBank accession number M17213.