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. 2020 Feb 21;9(2):38. doi: 10.3390/biology9020038

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Micrographs of nitrogen starved P. tricornutum cells, illustrating potential interconnectivity between LDs and various cellular compartments. Plastidial autofluorescence appears red, the LD stain Nile Red fluoresces yellow and the ER/mitochondrial/endomembrane stain DiOC₆ fluoresces green/greenish blue. (A) Epifluorescent image of cells stained with Nile Red and DiOC₆, (B) Epifluorescent image of cells stained with only DiOC₆, (C) Epifluorescent image of cells stained with Nile Red and DiOC₆, (D) Differential interference contrast image with no epifluorescent staining. P = plastid, L = lipid droplet, LPI = lipid droplet-plastid interface, LEI = lipid droplet-endomembrane interface. The interfacial regions, emphasized in boxes with dotted lines, are speculated to be potential regions of interaction between LDs and other organelles.