Table 1.
Proposed functions and homologies of the proteins encoded in the hum cluster.
| Proteins from hum Cluster | Closest BLAST Homolog (Organism) Reference (% Identity/% Similarity) |
Homologies with Other Siamycin-Like Clusters (% Identity/ % Similarity) |
Proposed Function | ||
|---|---|---|---|---|---|
| abo cluster | msl cluster | spe cluster | |||
| HumA | Aborycin family tricyclic lasso peptide (Streptomyces sp. F-7) WP_107308646.1 (95/100) |
(92.9/100) | (88.1/97.6) | (86.4/93.2) | Structural gene |
| HumB1 | Lasso peptide biosynthesis PqqD family chaperone (Streptomyces) WP_048459499.1 (77/86) |
(77/87.4) | (73.6/87.4) | (71.3/86.2) | RiPP recognition element (RRE) protein |
| HumB2 | Lasso peptide biosynthesis B2 protein (Streptomyces xanthocidicus) WP_117490375.1 (88/92) |
(84.4/90.1) | (86/91.6) | (88.8/91.6) | Leader peptidase |
| HumC | Lasso peptide isopeptide bond-forming cyclase (Streptomyces sp. CB02056) WP_074004732.1 (81/87) |
(76.1/84.5) | (76/82.1) | (76.3/84) | Lasso peptide cyclase |
| HumD1 | ATP-binding, cassette domain-containing protein (Streptomyces yerevanensis) WP_033320332.1 (86/90) |
(73.1/84.7) | (71.4/81) | (78.3/86) | Transporter |
| HumD2 | ABC transporter permease (Streptomyces scabiei) WP_037695288.1 (92/94) |
(79.6/87.6) | (78.1/88.4) | (72.4/82.4) | Transporter |
| HumD3 | ATP-binding, cassette domain-containing protein (Streptomyces xanthocidicus) WP_117490377.1 (83/90) |
(81.9/90.3) | (81/88.5) | (84.7/91.3) | Transporter |
| HumD4 | Multispecies: ABC transporter permease subunit (Streptomyces) WP_095851496.1 (87/91) |
(82.1/89.7) | (85.9/91.8) | (82.1/88) | Transporter |
| HumE | DoxX family membrane protein (Streptomyces specialis) WP_059005885.1 (71/80) |
(66.1/75.4) | (66.5/74.9) | (63.8/72.4) | Disulfide oxidoreductase |
| HumF | Thioredoxin domain-containing protein (Streptomyces xanthocidicus) WP_117490379.1 (76/85) |
(67.2/76.8) | (70.9/79.9) | (67.9/75.6) | Disulfide oxidoreductase |
| HumG | Two-component system sensor kinase (Streptomyces sp. NL15-2K) GCB43196.1 (79/87) |
(72.5/82) | (73.6/81.2) | (69.5/77.7) | Regulator |
| HumH | Multispecies: CapA family protein (Streptomyces) WP_074004738.1 (81/86) |
(73.4/83) | (74.1/83.6) | (75.9/83.4) | Putative epimerase |
| HumR1 | Response regulator transcription factor (Streptomyces sp. CB02056) WP_079272571.1 (75/86) |
(63.1/78.1) | (66.5/79.6) | (71.4/82.3) | Regulator |
| HumR2 | Multispecies: response regulator transcription factor (Streptomyces) WP_046710140.1 (93/97) |
(84.4/92) | (87.9/93.3) | (84.8/92.9) | Regulator |